With USB Sound Card Plugged Into Pi, Run The Following Command:

alsamixer

Click fn And F6 To List Audio Devices And Select The USB 

Adjust The Volume For Headphone And Mic
Turn Off Auto Gain By Clicking "m" On The Keyboard
Click "esc" On The Keyboard To Exit

Run The Following Command To Save Audio Settings:

sudo alsactl store

Run The Following Command To Get The "Card" And "Device" Numbers:

arecord -l

As You Can See In The 3rd Image That The Card Is "2" And The Device Is "0"
If You Run The Following Command It Will Show All Audio Devices:

aplay -l

Now Add The Following To The End Of The Command Like It Shows Below. Make Sure To Edit The Numbers If Needed

-O hw:2,0

If You Run Into Any Problems Try To Do The Following:

-O plughw:2,0

Now That We Have The USB Sound Card Setup And Speaker Plugged Into The USB Sound Card. We Need To Add A Option To The OP25 Command To Pipe The Audio Out The USB.

Not cd Into The APPS Dir:

cd op25/op25/gr-op25_repeater/apps/

./rx.py --args 'rtl' -N 'LNA:47' -S 2500000 -x 2 -f 495.600e6 -o 17e3 -q 0 -T trunk.tsv -V -2 -U 2> stderr-stream0.2 -O hw:2,0